imac wash buffer 1 (Bio-Rad)
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imac wash buffer 1
Imac Wash Buffer 1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 22183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imac+wash+buffer+1/Tris/us12422369-975-47-69
Average 98 stars, based on 22183 article reviews
Imac Wash Buffer 1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 22183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imac+wash+buffer+1/Tris/us12422369-975-47-69
Average 98 stars, based on 22183 article reviews
imac wash buffer 1 - by Bioz Stars,
2026-09
98/100 stars
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Affinity Chromatography:Article Title: Benzothiophene, thienopyridine and thienopyrimidine derivatives for the modulation of sting Article Snippet: Following expression, cell cultures were centrifuged at 5000×g for 20 min and cell pellet stored frozen at −70° C. Protein purification was initiated by thawing the cell pellet in Lysis buffer (25 mM Tris-HCl pH 8.0, 300 mM NaCl, 5 mM DTT, 2 mM MgCl2, 10 mM imidazole, 0.5 mg/ml lysozyme, benzonase endonuclease [EMD Millipore], 1 mM PMSF, complete protease inhibitor tablets EDTA-free [Roche]) using a ratio of 7 ml of buffer per 1 g of cells. .. Cells were further lysed by 3 passes through an ice cooled Avestin C5 cell crusher and then centrifuged at 48,000×g at 4° C. Supernatant (cell lysate) was filtered through a 5 μm filter and loaded onto 5 mL HiTrap IMAC Sepharose FF column (GE Healthcare) pre-equilibrated with Article Title: Immunosensor Article Snippet: Following expression, cell cultures were centrifuged at 5000×g for 20 min and cell pellets were stored frozen at −20° C. Protein Purification: Protein purification was initiated by thawing the cell pellet in Lysis buffer (25 mM Tris-HCl pH 8.0, 300 mM NaCl, 2 mM MgCl2, 10 mM Imidazole, 0.5 mg/mL lysozyme, 5 U/mL benzonase endonuclease [EMD Millipore], 1 mM PMSF, complete protease inhibitor tablets EDTA-free [Roche]) using a ratio of 5 mL of buffer per 1 g of cells. .. Cells were further lysed by 3 passes through an ice cooled Avestin C5 cell crusher and centrifuged at 48,000×g at 4° C. Supernatant (cell lysate) was filtered through a 5 μm filter and applied onto a 5 mL HiTrap IMAC Sepharose FF column (GE Healthcare) pre-equilibrated with Article Title: Immunosensor Article Snippet: Following expression, cell cultures were centrifuged at 5000×g for 20 min and cell pellets stored frozen at −20° C. Protein Purification: Protein purification was initiated by thawing the cell pellet in Lysis buffer (25 mM Tris-HCl pH 8.0, 300 mM NaCl, 2 mM MgCl2, 10 mM Imidazole, 0.5 mg/mL lysozyme, 5 U/mL benzonase endonuclease [EMD Millipore], 1 mM PMSF, complete protease inhibitor tablets EDTA-free [Roche]) using a ratio of 5 mL of buffer per 1 g of cells. .. Cells were further lysed by 3 passes through an ice cooled Avestin C5 cell crusher and centrifuged at 48,000×g at 4° C. Supernatant (cell lysate) was filtered through a 5 μm filter and applied onto a 1 mL HiTrap IMAC Sepharose FF column (GE Healthcare) pre-equilibrated with Article Title: Compound modulators of sting Article Snippet: Cells were further lysed by 3 passes through an ice cooled Avestin C5 cell crusher and then centrifuged at 48,000×g at 4° C. Supernatant (cell lysate) was filtered through a 5 μm filter and loaded onto 5 mL HiTrap IMAC Sepharose FF column (GE Healthcare) pre-equilibrated with IMAC wash buffer 1 (25 mM Tris-HCl pH 8.0, 300 mM NaCl, 5 mM DTT, 10 mM imidazole) using Profinia Affinity chromatography purification system (Bio-Rad). .. Cells were further lysed by 3 passes through an ice cooled Avestin C5 cell crusher and then centrifuged at 48,000×g at 4° C. Supernatant (cell lysate) was filtered through a 5 μm filter and loaded onto 5 mL HiTrap IMAC Sepharose FF column (GE Healthcare) pre-equilibrated with Article Title: Compounds Article Snippet: Following expression, cell cultures were centrifuged at 5000×g for 20 min and cell pellet stored frozen at −20° C. Protein Purification: Protein purification was initiated by thawing the cell pellet (34 g wet weight) in Lysis buffer (20 mM Hepes pH 7.5, 500 mM NaCl, 5 mM Imidazole, 5% [v/v]glycerol, 0.01% [v/v] Triton-X 100, 5 mM 2-mercaptoethanol, 2 mM MgCl2, 0.5 mg/mL lysozyme, benzonase endonuclease [EMD Millipore], 1 mM PMSF, complete protease inhibitor tablets EDTA-free [Roche]) using a ratio of 3 mL of buffer per 1 g of cells. .. Cells were further lysed by 3 passes (at 15000 psi) through an ice cooled Avestin C5 cell crusher and then centrifuged at 48,000×g at 4° C. Supernatant (cell lysate) was filtered through a 0.2 μm filter and applied onto 5 mL HiTrap IMAC Sepharose FF column (GE Healthcare) pre-equilibrated with Article Title: Immunosensor Article Snippet: Following expression, cell cultures were centrifuged at 5000×g for 20 min and the cell pellet stored frozen at −20° C. Protein Purification: Protein purification was initiated by thawing the cell pellet in Lysis buffer (25 mM Tris-HCl pH 8.0, 300 mM NaCl, 2 mM MgCl2, 10 mM Imidazole, 0.5 mg/ml lysozyme, benzonase endonuclease [EMD Millipore], 1 mM PMSF, complete protease inhibitor tablets EDTA-free [Roche]) using a ratio of 5 ml of buffer per 1 g of cells. .. Cells were further lysed by 3 passes through an ice cooled Avestin C5 cell crusher and then centrifuged at 48,000×g at 4° C. Supernatant (cell lysate) was filtered through a 5 μm filter and applied to 1 ml HiTrap IMAC Sepharose FF column (GE Healthcare) pre-equilibrated with Article Title: Immunosensor Article Snippet: Cultures were transferred to 16° C., protein expression was induced by the addition of isopropyl β-D-1-thiogalactopyranoside (IPTG) to a final concentration of 0.5 mM and the cultures were shaken overnight for a further 16 h. Following expression, cell cultures were centrifuged at 5000×g for 20 min and the resulting cell pellets were stored frozen at −20° C. Protein Purification: Protein purification was initiated by thawing the cell pellet in Lysis buffer (25 mM Tris-HCl pH 8.0, 300 mM NaCl, 2 mM MgCl2, 10 mM imidazole, 0.5 mg/ml lysozyme, 5 U/ml benzonase endonuclease [EMD Millipore], 1 mM PMSF, complete protease inhibitor tablets EDTA-free [Roche]) using a ratio of 5 ml of buffer per 1 g of cells. .. Cells were further lysed by 3 passes through an ice cooled Avestin C5 cell crusher and centrifuged at 48,000×g at 4° C. Supernatant (cell lysate) was filtered through a 5 μm filter and applied onto a 5 ml HiTrap IMAC Sepharose FF column (GE Healthcare) pre-equilibrated with Purification:Article Title: Benzothiophene, thienopyridine and thienopyrimidine derivatives for the modulation of sting Article Snippet: Following expression, cell cultures were centrifuged at 5000×g for 20 min and cell pellet stored frozen at −70° C. Protein purification was initiated by thawing the cell pellet in Lysis buffer (25 mM Tris-HCl pH 8.0, 300 mM NaCl, 5 mM DTT, 2 mM MgCl2, 10 mM imidazole, 0.5 mg/ml lysozyme, benzonase endonuclease [EMD Millipore], 1 mM PMSF, complete protease inhibitor tablets EDTA-free [Roche]) using a ratio of 7 ml of buffer per 1 g of cells. .. Cells were further lysed by 3 passes through an ice cooled Avestin C5 cell crusher and then centrifuged at 48,000×g at 4° C. Supernatant (cell lysate) was filtered through a 5 μm filter and loaded onto 5 mL HiTrap IMAC Sepharose FF column (GE Healthcare) pre-equilibrated with Article Title: Immunosensor Article Snippet: Following expression, cell cultures were centrifuged at 5000×g for 20 min and cell pellets were stored frozen at −20° C. Protein Purification: Protein purification was initiated by thawing the cell pellet in Lysis buffer (25 mM Tris-HCl pH 8.0, 300 mM NaCl, 2 mM MgCl2, 10 mM Imidazole, 0.5 mg/mL lysozyme, 5 U/mL benzonase endonuclease [EMD Millipore], 1 mM PMSF, complete protease inhibitor tablets EDTA-free [Roche]) using a ratio of 5 mL of buffer per 1 g of cells. .. Cells were further lysed by 3 passes through an ice cooled Avestin C5 cell crusher and centrifuged at 48,000×g at 4° C. Supernatant (cell lysate) was filtered through a 5 μm filter and applied onto a 5 mL HiTrap IMAC Sepharose FF column (GE Healthcare) pre-equilibrated with Article Title: Immunosensor Article Snippet: Following expression, cell cultures were centrifuged at 5000×g for 20 min and cell pellets stored frozen at −20° C. Protein Purification: Protein purification was initiated by thawing the cell pellet in Lysis buffer (25 mM Tris-HCl pH 8.0, 300 mM NaCl, 2 mM MgCl2, 10 mM Imidazole, 0.5 mg/mL lysozyme, 5 U/mL benzonase endonuclease [EMD Millipore], 1 mM PMSF, complete protease inhibitor tablets EDTA-free [Roche]) using a ratio of 5 mL of buffer per 1 g of cells. .. Cells were further lysed by 3 passes through an ice cooled Avestin C5 cell crusher and centrifuged at 48,000×g at 4° C. Supernatant (cell lysate) was filtered through a 5 μm filter and applied onto a 1 mL HiTrap IMAC Sepharose FF column (GE Healthcare) pre-equilibrated with Article Title: Compound modulators of sting Article Snippet: Cells were further lysed by 3 passes through an ice cooled Avestin C5 cell crusher and then centrifuged at 48,000×g at 4° C. Supernatant (cell lysate) was filtered through a 5 μm filter and loaded onto 5 mL HiTrap IMAC Sepharose FF column (GE Healthcare) pre-equilibrated with IMAC wash buffer 1 (25 mM Tris-HCl pH 8.0, 300 mM NaCl, 5 mM DTT, 10 mM imidazole) using Profinia Affinity chromatography purification system (Bio-Rad). .. Cells were further lysed by 3 passes through an ice cooled Avestin C5 cell crusher and then centrifuged at 48,000×g at 4° C. Supernatant (cell lysate) was filtered through a 5 μm filter and loaded onto 5 mL HiTrap IMAC Sepharose FF column (GE Healthcare) pre-equilibrated with Article Title: Compounds Article Snippet: Following expression, cell cultures were centrifuged at 5000×g for 20 min and cell pellet stored frozen at −20° C. Protein Purification: Protein purification was initiated by thawing the cell pellet (34 g wet weight) in Lysis buffer (20 mM Hepes pH 7.5, 500 mM NaCl, 5 mM Imidazole, 5% [v/v]glycerol, 0.01% [v/v] Triton-X 100, 5 mM 2-mercaptoethanol, 2 mM MgCl2, 0.5 mg/mL lysozyme, benzonase endonuclease [EMD Millipore], 1 mM PMSF, complete protease inhibitor tablets EDTA-free [Roche]) using a ratio of 3 mL of buffer per 1 g of cells. .. Cells were further lysed by 3 passes (at 15000 psi) through an ice cooled Avestin C5 cell crusher and then centrifuged at 48,000×g at 4° C. Supernatant (cell lysate) was filtered through a 0.2 μm filter and applied onto 5 mL HiTrap IMAC Sepharose FF column (GE Healthcare) pre-equilibrated with Article Title: Immunosensor Article Snippet: Following expression, cell cultures were centrifuged at 5000×g for 20 min and the cell pellet stored frozen at −20° C. Protein Purification: Protein purification was initiated by thawing the cell pellet in Lysis buffer (25 mM Tris-HCl pH 8.0, 300 mM NaCl, 2 mM MgCl2, 10 mM Imidazole, 0.5 mg/ml lysozyme, benzonase endonuclease [EMD Millipore], 1 mM PMSF, complete protease inhibitor tablets EDTA-free [Roche]) using a ratio of 5 ml of buffer per 1 g of cells. .. Cells were further lysed by 3 passes through an ice cooled Avestin C5 cell crusher and then centrifuged at 48,000×g at 4° C. Supernatant (cell lysate) was filtered through a 5 μm filter and applied to 1 ml HiTrap IMAC Sepharose FF column (GE Healthcare) pre-equilibrated with Article Title: Immunosensor Article Snippet: Cultures were transferred to 16° C., protein expression was induced by the addition of isopropyl β-D-1-thiogalactopyranoside (IPTG) to a final concentration of 0.5 mM and the cultures were shaken overnight for a further 16 h. Following expression, cell cultures were centrifuged at 5000×g for 20 min and the resulting cell pellets were stored frozen at −20° C. Protein Purification: Protein purification was initiated by thawing the cell pellet in Lysis buffer (25 mM Tris-HCl pH 8.0, 300 mM NaCl, 2 mM MgCl2, 10 mM imidazole, 0.5 mg/ml lysozyme, 5 U/ml benzonase endonuclease [EMD Millipore], 1 mM PMSF, complete protease inhibitor tablets EDTA-free [Roche]) using a ratio of 5 ml of buffer per 1 g of cells. .. Cells were further lysed by 3 passes through an ice cooled Avestin C5 cell crusher and centrifuged at 48,000×g at 4° C. Supernatant (cell lysate) was filtered through a 5 μm filter and applied onto a 5 ml HiTrap IMAC Sepharose FF column (GE Healthcare) pre-equilibrated with other:Article Title: Compounds Article Snippet: KAT8 Molecular Biology: A codon optimized DNA sequence (for expression in E. coli) encoding amino acid residues 177 to 447 (Uniprot Q9H7Z6-1) of human KAT8 was synthesised by Thermo Fisher Scientific GENEART GmbH (Regensberg, Germany). |